Fig. 1. Time-dependent uptake of the 12 OATP substrates in cryopreserved rat hepatocyte suspension. Uptake of rosuvastatin (A), fluvastatin (B), cerivastatin (C), pravastatin (D), atorvastatin (E), pitavastatin (F), bosentan (G), glyburide (H), nateglinide (I), valsartan (J), asunaprevir (K), and telmisartan (L) at 1 µM was determined in the regular KHB (left column) or KHB containing 4% BSA (right column) for (20 seconds to 30 minutes) at 37°C (closed circle), 37°C in the presence of 1 mM rifamycin SV (open circle), or 4°C (closed triangle). The uptake velocity (V0) was calculated by dividing the amount of compound measured in rat hepatocytes at each time point by the total compound concentration in the reaction buffer in the absence or presence of 4% BSA. Data represent duplicate measurement at each time points.