Fig. 9. Comparison of representative other seasonal PK parameters (AUCinf, Cmax, t1/2, Vss, and CL) between May 11 and November 23, 2017, in (A) males and (B) females. Data in green were from May 11, 2017, and data in red were from Nov 23, 2017. AUCinf and CL values were plotted in log scale, whereas other PK parameters were plotted in linear scale. Symbols: ∆, AP; X, RTG; ○, 3TC;, ICG. In both male and female woodchucks, AUCinf increases were statistically significant for AP (P < 0.0001 for male and P = 0.026 for female), RTG (P < 0.0001 for both male and female), and 3TC (P < 0.0001 for male and P = 0.041 for female) but not for ICG (P = 0.69 for male and P = 0.77 for female). No statistically significant Vss changes (P ≥ 0.22) were observed for any of the four compounds tested in either male or female woodchucks. In both male and female woodchucks, CL decreases were statistically significant for AP (P < 0.0001 for male and P = 0.0047 for female), RTG (P < 0.0001 for both male and female), and 3TC (P < 0.0001 for male and P = 0.016 for female) but not for ICG (P = 0.29 for male and P = 0.48 for female). In males, increases of Cmax were statistically significant for AP (P = 0.0017), RTG (P < 0.0001), and 3TC (P < 0.0001) but not for ICG (P = 0.68). In females, Cmax increases were statistically significant for ICG (P = 0.037) but not for AP (P = 0.14), RTG (P = 0.28), or 3TC (P = 0.36). In males, increases of t1/2 were statistically significant for AP (P < 0.0001) and 3TC (P = 0.0003) but not for RTG (P = 0.061) or ICG (P = 0.48). In females, t1/2 increases were statistically significant for AP (P = 0.039) but not for RTG (P = 0.30), 3TC (P = 0.14), or ICG (P = 0.70). Statistical analysis was performed using an unpaired, two-tailed, parametric t test method (mean ± S.D., n = 6 or 7 for male, n = 7 or 9 for female; three woodchucks died during the study).