Article Figures & Data
Figures
- Fig. 1.
Bile acid biosynthesis, transport, and metabolism. Ath r epresents "atherogenic diet" and DR respresents "diet restriction". The left arrow (solid line) shows the trend of male mice, and the right arrow (dashed line) shows the trend of female mice.
- Fig. 2.
Three-dimensional scatter plots of the PCA for the influence of different diets on liver BA profile and BA-related gene mRNA expression in both genders of mice. (A) PCA based on the BA-related gene mRNA expression in livers of male mice (R2 = 0.797, Q2 = 0.532). (B) PCA based on the BA-related gene mRNA expression in livers of female mice (R2 = 0.828, Q2 = 0.584). (C) PCA based on liver BA concentrations of male mice (R2 = 0.988, Q2 = 0.664). (D) PCA based on liver BA concentrations of female mice (R2 = 0.956, Q2 = 0.618). Each color represents a single diet. PCA was performed using the software SIMCA.
- Fig. 3.
Effect of nine diets on the concentrations of BAs in the serum of mice. (A) The amount of T-conjugated, unconjugated, and total BAs in the serum of mice were quantified. (B) Heatmap of BAs in the serum of mice fed nine various diets. Average values of five replicates per diet are given by colored squares. High BA concentration is represented in red, whereas low BA concentration is in blue. *P < 0.05 compared with control (AIN-93M purified diet) in male and female mice, respectively; #P < 0.05 compared between genders fed the same diet.
- Fig. 4.
Effect of nine diets on the concentrations of BAs in the livers of mice. (A) The amount of T-conjugated, unconjugated, and total BAs in the livers of mice were quantified. (B) Heatmap of BAs in the livers of mice fed nine various diets. Average values of five replicates per diet are given by colored squares. High BA concentration is represented in red, whereas low BA concentration is in blue. *P < 0.05 compared with control (AIN-93M purified diet) in male and female mice, respectively; #P < 0.05 compared between genders fed the same diet.
- Fig. 5.
Effect of nine diets on mRNA expression of BA-related genes in the livers and ileum of mice. Heatmap of BA-related genes in the livers and ileum of mice fed nine various diets. Average values of five replicates per diet are given by colored squares. High mRNA abundance is represented in red, whereas low mRNA abundance is in blue. Relative mRNA levels were calculated with male controls (male, AIN-93M purified diet) set as 100%. Data were analyzed by two-way ANOVA. *P < 0.05 compared with control (AIN-93M purified diet) in male and female mice, respectively; #P < 0.05 when data from the two genders fed the same diet are compared.
Tables
- TABLE 1
The ratio of 12α-OH BAs to non–12α-OH BAs in livers and serum of two genders
12α-OH BAs: CA, DCA, TCA, TDCA; non–12α-OH BAs: CDCA, LCA, UDCA, HDCA, MDCA, TCDCA, TLCA, TUDCA, TMDCA, THDCA, MCAs and TMCAs.
Diet Male Female Liver Serum Liver Serum AIN-93M purified 0.24 ± 0.05 0.53 ± 0.15 0.57 ± 0.05 1.55 ± 0.2 Laboratory chow 0.61 ± 0.1 0.69 ± 0.15 0.8 ± 0.1 1.28 ± 0.25 Diet-restriction 2.12 ± 0.55* 2.14 ± 0.6* 1.65 ± 0.15* 2.02 ± 0.35 High fructose 0.47 ± 0.45 0.33 ± 0.15 0.8 ± 0.05 1.5 ± 0.15 High fat 0.29 ± 0.05 0.5 ± 0.20 0.8 ± 0.25 1.51 ± 0.35 Atherogenic 30.09 ± 3.25* 48.47 ± 9.8* 21.73 ± 8.75* 41.24 ± 16.35* Western 0.26 ± 0.1 0.61 ± 0.2 0.3 ± 0.05 0.82 ± 0.2 Low n-3 FA 0.23 ± 0.05 0.56 ± 0.25 0.51 ± 0.2 1.53 ± 0.35 EFA deficient 0.28 ± 0.1 0.22 ± 0.1 0.56 ± 0.15 1.05 ± 0.3 ↵* Indicates P < 0.05 when compared with the AIN-93M purified diet. Mean values ± S.D. are shown.
Data Supplement
- Supplemental Tables -
Supplementary Table 1 - Standard curve data of the different bile acids.
Supplementary Table 2 - The effects of nine diets on serum individual, total, T-conjugated, unconjugated BAs.
Supplementary Table 3 - The effects of nine diets on liver individual, total, T-conjugated, unconjugated BAs.
Supplementary Table 4 - The effects of nine diets on BA-related genes in liver and ileum.
Supplementary Table 5 - The correlation analysis of BAs (total, conjugated and unconjugated) and BA-related genes involved in the synthesis, metabolism and transport of BAs in the livers of mice fed the 9 diets.
- Supplemental Tables -
