Abstract
Aldo-keto reductases (AKRs) are a class of NADPH-dependent oxidoreductases that have been linked to metabolism of the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). Although widely used, cardiotoxicity continues to be a serious side effect that may be linked to metabolites or reactive intermediates generated in their metabolism. In this study we examine the little known effects of nonsynonymous single nucleotide polymorphisms of human AKR1A1 on the metabolism of these drugs to their alcohol metabolites. Expressed and purified from bacteria using affinity chromatography, the AKR1A1 protein with a single histidine (6x-His) tag exhibited the greatest activity using two test substrates: p-nitrobenzaldehyde (5.09 ± 0.16 μmol/min/mg of purified protein) and dl-glyceraldehyde (1.24 ± 0.17 μmol/min/mg). These activities are in agreement with published literature values of nontagged human AKR1A1. The 6x-His-tagged AKR1A1 wild type and allelic variants, E55D and N52S, were subsequently examined for metabolic activity using DAUN and DOX. The tagged variants showed significantly reduced activities (1.10 ± 0.42 and 0.72 ± 0.47 nmol of daunorubicinol (DAUNol) formed/min/mg of purified protein for E55D and N52S, respectively) compared with the wild type (2.34 ± 0.71 nmol/min/mg). The wild type and E55D variant metabolized DOX to doxorubicinol (DOXol); however, the levels fell below the limit of quantitation (25 nM). The N52S variant yielded no detectable DOXol. A kinetic analysis of the DAUN reductase activities revealed that both amino acid substitutions lead to reduced substrate affinity, measured as significant increases in the measured Km for the reduction reaction by AKR1A1. Hence, it is possible that these allelic variants can act as genetic biomarkers for the clinical development of DAUN-induced cardiotoxicity.
Footnotes
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This study was supported by a grant from the Canadian Institutes of Health Research (CIHR). R.H.T. was supported by an Rx&D/CIHR scholarship. O.S.B. was supported by a University of British Columbia Graduate Fellowship.
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O.S.B. and R.H.T. contributed equally to the experimental studies and writing of this manuscript.
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Article, publication date, and citation information can be found at http://dmd.aspetjournals.org.
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doi:10.1124/dmd.107.018895.
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ABBREVIATIONS: AKR, aldo-keto reductase; DOX, doxorubicin; DAUN, daunorubicin; ns, nonsynonymous; SNP, single nucleotide polymorphism; DOXol, doxorubicinol; DAUNol, daunorubicinol; 6x-His, six histidine; HPLC, high-performance liquid chromatography; FXa, factor Xa; Ni-NTA, nickel-nitrilotriacetic acid; ANOVA, analysis of variance; GST, glutathione S-transferase; ROS, reactive oxygen species.
- Received September 19, 2007.
- Accepted February 12, 2008.
- The American Society for Pharmacology and Experimental Therapeutics
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