Abstract
Kakkalide is a major isoflavonoid of the flowers of Pueraria lobata (Willd.) Ohwi possessing the protective effect against ethanol-induced intoxication and hepatic injury. The metabolism of kakkalide was investigated in rat. Thirteen metabolites were isolated using solvent extraction and repeated chromatographic methods, and identified using spectroscopic methods including UV, IR, MS, NMR and CD experiments. Four new compounds were identified as irisolidone-7-O-glucuronide (M-1), tectorigenin-7-O-sulfate (M-2), tectorigenin-4'-O-sulfate (M-3) and biochanin A-6-O-sulfate (M-4), together with nine known compounds irisolidone (M-5), tectorigenin (M-6), tectoridin (M-7), 5,7-dihydroxy-8,4'-dimethoxyisoflavone (M-8), isotectorigenin (M-9), biochanin A (M-10), genistein (M-11), daidzein (M-12) and equol (M-13). The metabolic pathway of kakkalide was proposed, which is important to understand its metabolic fate and disposition in human.
Footnotes
- Received June 3, 2009.
- Accepted November 5, 2009.
- The American Society for Pharmacology and Experimental Therapeutics