Article Figures & Data
Additional Files
Data Supplement
- Supplemental Data -
Supplementary Figure 1 - Representative MRM chromatograms for DOXO and metabolites. (A) DOXO solution (100 ng/mL); (B) M1 solution (100 ng/mL); (C) M2 solution (100 ng/mL); (D) M4 solution (100 ng/mL); (E) M5 solution (100 ng/mL); (F) HLM incubation, blank sample (without DOXO); (G) HLM incubation, blank sample spiked with mixture of DOXO, M1, M2, M4 and M5 (final concentration: 100 ng/ml each); (H) HLS9 incubation, blank sample (without DOXO) (I) HLS9 incubation, blank sample spiked with mixture of DOXO, M1, M2, M4 and M5 (final concentration: 100 ng/ml each); (J) Plasma, blank sample (non-DOXO treated), (K) Plasma, blank sample spiked with mixture standards of DOXO, M1, M2, M4 and M5 (final plasma concentration: 100 ng/ml each).
Supplementary Figure 2 - Chromatography - Mass Spectrometry of DOXO obtained on LC Triple TOF. (A) Chromatographic graph of DOXO; (B) MS2 spectrum of DOXO in the positive ion mode; (C) Tentative structural illustration of the most informative fragment ions for DOXO.
Supplementary Figure 3 - The MS2 spectra of representative metabolites in HLM. (A) M1; (B) M2; (C) M3; (D) M4; (E) M5; (F) M9; (G) M6; (H) M7 and M8.
Supplementary Figure 4 - Chromatography of purity test for reference standards used in the study. (A) DOXO; (B) M1; (C) M2; (D) M3; (E) M4; (F) M5.
Supplementary Figure 5 - The MS2 spectra for reference standards obtained on LC-Triple TOF (A) M1; (B) M2; (C) M3; (D) M4; (E) M5.
Supplementary Figure 6 - The 1H-NMR for reference standards. (A) DOXO; (B) M1; (C) M2; (D) M3; (E) M4; (F) M5.
Supplementary Figure 7 - The 13C-NMR for reference standards. (A) DOXO; (B) M1; (C) M2; (D) M3; (E) M4; (F) M5.
Supplementary Figure 8 - TIC chromatogram of different substrates in phase II metabolism incubation system in the presences or absence of HLM determined by LC-Triple TOF. (A) DOXO; (B) M2; (C) M4.
Supplementary Table 1 - The ion transitions monitored for DOXO and metabolites.
Supplementary Table 2 - Metabolites of DOXO detected by LC-Triple TOF in HLM.
Supplementary Table 3 - The HPLC condition for purity test.
Supplementary Table 4 - Representative calibration curves for DOXO, M1, M2, M4, and M5.
- Supplemental Data -