Article Figures & Data
Additional Files
Data Supplement
- Supplemental Data -
Supplemental Table S1. Characteristics and demographic details of the studied hepatocytes.
Supplemental Table S2. Demographics of the studied clinical subjects.
Supplemental Table S3. Acquisition parameters of Full MS/data-dependent (dd)-MS2 in Thermo Scientific™ Q Exactive™ HF mass spectrometry.
Supplemental Table S4. Chromatographic conditions for the separation of proteotypic UGT peptides.
Supplemental Table S5. List of selected prototype peptides for targeted UGT enzyme quantification and multiple reaction monitoring (MRM) parameters for LC-MS/MS analysis.
Supplemental Table S6. Untargeted metabolomics analysis of representative human serum sample at respective pre-dose and post-dose (following oral administration of 100 mg DMAU).
Supplemental Table S7. Protein abundance and relative expression factor (REF) of UGT1A9 and UGT2B17 in different subcellular fractions of intestine and liver*
Supplemental Figure S1. Representative ion chromatograms of DMA and DMA-G in (A) human hepatocyte and (B) serum samples.
Supplemental Figure S2. The metabolite (DMA-G and androstenedione analogues) to DMA ratios formed in
human hepatocytes at 1 μM and 10 μM DMAU (red) and DMA (blue) concentrations at 60 and 120 min incubations.Supplemental References.
- Supplemental Data -