Data Supplement

• Supplemental Data -

  Supplemental Table S1. siRNA sequences for target genes.

  Supplemental Table S2. Primer sequences for RT-qPCR.

  Supplemental Table S3. Transitions of target analytes including ISTD by LC-MS/MS system.

  Supplemental Figure S1. The transfection efficiency of small interfering RNA silencing or overexpression plasmids in mouse primary hepatocytes or HepG2 cells.

  Supplemental Figure S2. Interrelationships among SLC13A5, SLC25A1, and ACLY in mouse primary hepatocytes treated with pharmacological inhibitors and HepG2 cells treated with siRNA.

  Supplemental Figure S3. The inhibitory potency of bempedoic acid on the extracellular citrate uptake in HEK293T and HepG2 cells transfected with SLC13A5.

  Supplemental Figure S4. Bempedoic acid reduces lipid deposition in mouse primary hepatocytes and HepG2 cells challenged with high energy.

  Supplemental Figure S5. The levels of bempedoic acid, ESP 15228 and the related lipogenic metabolites determined in the plasma and liver tissue of HFHFD-fed mice.

  Supplemental Figure S6. Bempedoic acid downregulates both ACLY and SLC13A5 via suppressing PXR activation in HepG2 cells.