Data Supplement

• Supplemental Figures -

  Supplemental Figure S1. The expression of SLC19A1 and SLC46A1 in human tissues and NSCLC.

  Supplemental Figure S2. BCRP and MRP5 expressions in NSCLC cells.

  Supplemental Figure S3. Effect of pH on the growth of H1299, H1650, H1975 and A549 cells.

  Supplemental Figure S4. (A) Histological analysis was performed on kidney and liver sections collected from H1650-vector, H1650-RFC and H1650-PCFT xenografts. H&E staining shows neither nephrotoxicity nor hepatotoxicity in mice undergoing pemetrexed treatment. Scale bar = 50 μm. PEM: pemetrexed (B) Body weight curves for H1650-vector, H1650-RFC and H1650-PCFT xenografts.

  Supplemental Figure S5. pH-dependent accumulation of pemetrexed in RFCexpressing cells for 30 min.