Data Supplement

• Supplemental Figures -

  Supplemental Figure S1. a) Horizontal stacked bar charts illustrating the relative abundances of hepatic CYPs 20A1, 27A1, 51A1, 7B1, and 8B1 in in 58 cryopreserved human hepatocyte samples (age, 1 day- 63 years). The left side of the charts displays the data categorized by age, while the right side represents the data categorized by total abundance of all proteins in each donor. b) Categorical data for CYPs, 20A1, 27A1, 51A1, 7B1, and 8B1 in pediatric (n=50) and adult hepatocytes (n=8). The truncated violin plot indicates the range, median, 25th and 75th percentiles. 

  Supplemental Figure S2. Categorical data for calnexin (ER membrane marker) in in 58 cryopreserved human hepatocytes (age, 1 day- 63 years).

  Supplemental Figure S3. Continuous age-dependent protein abundance of hepatic CYP enzymes in 58 cryopreserved human hepatocytes (age, 1 day- 63 years).

  Supplemental Figure S4. Sex-dependent differences in the abundances of hepatic CYPs1A2, 2A6, 2B6, 2C8, 2C9, 2C18, 2C19, 2D6, 2J2, 2E1, 3A4, 3A5, 3A7, 4A11, 4F2, 4F3, 4F11, 4F12, 4V2, 27A1, 51A1, 7B1, 8B1, and calnexin in cryopreserved human hepatocytes (>1 yr of age samples).

  Supplemental Figure S5. a) Peak intensities of 4-hydroxy diclofenac across different age groups of pediatric and adult hepatocytes b) Correlation of CYP2C9 abundance and activity in pediatric (n=50) and adult hepatocytes (n=8).
  

• Supplemental Tables -

  Supplemental Table S1. Demographic information of hepatocyte samples.

  Supplemental Table S2. Chromatographic conditions used for the analysis of 4-hydroxydiclofenac formation activity in pediatric and adult hepatocytes.

  Supplemental Table S3. MS method used for the analysis of 4-hydroxydiclofenac in pediatric and adult hepatocytes.

  Supplemental Table S4. Average abundance of hepatic CYP enzymes in different age-groups.

  Supplemental Table S5. Parameters describing the ontogeny profile of hepatic CYP enzymes.