Supplemental Data
Files in this Data Supplement:
- Supplemental Figures -
Supplemental Figure 1 - Expression of P-gp in mouse cell lines determined by Simple Western analysis
Supplemental Figure 2 - Flow cytometry analysis of substrate accumulation in human and mouse parental and ABCG2-expressing cells
Supplemental Figure 3 - Expression of ABCG2 and P-gp in human cell lines determined by Simple Western analysis
Supplemental Figure 4 - Sequencing data for 3T3, 3T3 MX15, Ltk- and Ltk- HoeR415 showed wildtype sequence (R482, underlined) with no trace of secondary mutant DNA signatures
Supplemental Figure 5 - Rhodamine 123 is not a mouse ABCG2 substrate
Supplemental Figure 6 - Rhodamine 123 efflux in mouse ABCG2-expressing subline is due to P-gp
Supplemental Figure 7 - Flow cytometry data of known weak substrates of P-gp in mouse ABCG2-expressing subline in the presence of a P-gp inhibitor
Supplemental Figure 8 - Flow cytometry of purpurin-18 accumulation in the presence of the following inhibitors in mouse and human ABCG2-expressing sublines demonstrated a similar pattern of inhibition
Supplemental Figure 9 - Flow cytometry data of purpurin-18 accumulation in the presence of inhibitors at 10 muM demonstrated a similar pattern of inhibition in human and mouse ABCG2-expressing cell lines
Supplemental Figure 10 - Flow cytometry data of BODIPY-prazosin accumulation in the human ABCG2- expressing subline H460 MX20