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Research ArticleArticle

Optimized Methods for Targeted Peptide-based Quantification of Human Uridine 5'-Diphosphate-glucuronosyltransferases in Biological Specimens Using Liquid Chromatography-tandem Mass Spectrometry

Yuichiro Sato, Masanori Nagata, Kazuhiro Tetsuka, Kouichi Tamura, Aiji Miyashita, Akio Kawamura and Takashi Usui
Drug Metabolism and Disposition March 4, 2014, dmd.113.056291; DOI: https://doi.org/10.1124/dmd.113.056291
Yuichiro Sato
1 Astellas Pharma Inc.;
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  • For correspondence: yuichiro.sato@astellas.com
Masanori Nagata
1 Astellas Pharma Inc.;
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Kazuhiro Tetsuka
2 Astellas Research Institute of America LLC.
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Kouichi Tamura
2 Astellas Research Institute of America LLC.
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Aiji Miyashita
1 Astellas Pharma Inc.;
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Akio Kawamura
1 Astellas Pharma Inc.;
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Takashi Usui
1 Astellas Pharma Inc.;
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Data Supplement

Files in this Data Supplement:

  • Data Supplement -

    Table S-1 - Sequences, MRM transitions, and retention times of analytes and IS

    Figure S-1 - Chromatograms of the analytes in digested rhUGTs

    Figure S-2 - Comparison of the efficiency for generating proteotypic peptides from biological specimens

    Figure S-3 - Production of UGT-proteotypic peptides in individual HLM

    Figure S-4 - Production of UGT-proteotypic peptides in individual HIM, pooled HKM (lot 1210288) and rhUGTs

  View article

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