Data Supplement
Files in this Data Supplement:
- Supplemental Data -
Supplemental Table 1 - Percent recovery of 14Claromustine from incubations with rat, dog, monkey and human liver microsomes (1 mg protein/mL) in the presence and absence of NADPH
Supplemental Table 2 - Conversion of 14Claromustine (100 μM) to radioactive components (C-1, C-2, C-3, C-4 and C-7) by rat, dog, monkey and human liver microsomes (1 mg protein/mL) in the presence and absence of NADPH
Supplemental Table 3 - Percent conversion of 14Claromustine (100 μM) to radioactive components (C-1, C-2, C-3, C-4 and C-7) by rat, dog, monkey and human liver microsomes (1 mg protein/mL) in the presence and absence of NADPH
Supplemental Table 4 - Formation of C-7 and loss of substrate in incubations of 14Claromustine (25 and 100 ?M) with various recombinant human CYP enzymes (25 pmol/incubation) expressed in insect cells (supersomes)
Supplemental Table 5 - Analysis of the correlation between the rate of formation of C-7 from 14Claromustine (25 ?M) and marker CYP and FMO activities in a bank of human liver microsomes (n = 10)
Supplemental Table 5 - Individual and Mean Plasma Concentrations and Pharmacokinetics of Total Radioactivity Following Intravenous Bolus Dose Administration of 10 mg/kg 14Claromustine to Group 3 Rats
Supplemental Table 7 - Reactive moieties originating from laromustine and VNP4090CE
Supplemental Figure 1 - LC-MS spectrum of M+NH4+, m/z 325, laromustine in LTQ (A) Full scan, (B) MS2, (C) MS3, (D) MS4 and (E) MS5 (Reproduced from Nassar et al., Chapter 5, ?Biotransformation and metabolite elucidationof xenobiotics? 2010)
Supplemental Figure 2 - Representative LC-MS spectra of M+NH4+, m/z 232, C-3 (A) Full Scan, (B) MS2 and (C) MS3 (Reproduced from Nassar et al., Chapter 6, ?Biotransformation and metabolite elucidationof xenobiotics? 2010)
Supplemental Figure 3 - Representative LC-MS spectra of M+NH4+, m/z 232, C-4 (A) Full Scan, (B) MS2 and (C) MS3 (Reproduced from Nassar et al., Chapter 6, ?Biotransformation and metabolite elucidationof xenobiotics? 2010)
Supplemental Figure 4 - Proposed structure and fragmentation ions for C-4 (Reproduced from Nassar et al., Chapter 6, ?Biotransformation and metabolite elucidationof xenobiotics? 2010)
Supplemental Figure 5 - Proposed structure and fragmentation ions for C-5 (Reproduced from Nassar et al., Chapter 6, ?Biotransformation and metabolite elucidationof xenobiotics? 2010)
Supplemental Figure 6 - LC-MS spectra of m/z 414, M-3 (A) full scan MS, (B) MS2, (C) MS3, (D) MS4, and (E) MS5 (Reproduced from Nassar et al., Chem. Res. Toxicol, 2011)
Supplemental Figure 7 - Proposed structure and fragmentation pattern for M-3 (Reproduced from Nassar et al., Chem. Res. Toxicol, 2011)