Applying Stable Isotope Labeled Amino Acids in Micropatterned Hepatocyte Co-Culture to Directly Determine the Degradation Rate Constant for CYP3A4

Data Supplement

Files in this Data Supplement:

• Supplemental Data -

  Supplemental Table 1 - Donor information for hepatocytes used for HepatoPac cultures

  Supplemental Table 2 - Summary of tryptic peptides and their selected reaction-monitoring parameters used for LC-MS/MS protein measurements

  Supplemental Table 3 - CYP3A4 stability data (mRNA, protein expression, and enzymatic activity) for four HepatoPac donors for Days 10-13 of SILAC culture

  Supplemental Table 4 - Calculated t1/2 (h) and kdeg (h-1) values from individual donors for CYP3

  Supplemental Figure 1 - Representative SRM chromatograms showing CYP3A4 peptide, SLLSPTFTSGK, for one HepatoPac donor over Days 10-13 with SILAC