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Research ArticleArticle

Global Proteomic Analysis of Human Liver Microsomes: Rapid Characterization and Quantification of Hepatic Drug-Metabolizing Enzymes

Brahim Achour, Hajar Al-Feteisi, Francesco Lanucara, Amin Rostami-Hodjegan and Jill Barber
Drug Metabolism and Disposition April 3, 2017, dmd.116.074732; DOI: https://doi.org/10.1124/dmd.116.074732
Brahim Achour
1 University of Manchester;
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Hajar Al-Feteisi
1 University of Manchester;
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Francesco Lanucara
2 Waters Corporation
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Amin Rostami-Hodjegan
1 University of Manchester;
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Jill Barber
1 University of Manchester;
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  • For correspondence: jill.barber@manchester.ac.uk

Data Supplement

Files in this Data Supplement:

  • Supplemental Data -

    Supplemental Methods

    Supplemental Table 1 - An outline of the methodological steps constituting the quantitative proteomic workflows specific to each methodology used for quantitative analysis

    Supplemental Figure 1 - Experimental workflow used in the label-free proteomic analysis of HLM samples

    Supplemental Figure 2 - Protein concentration in HLM samples (A), and the amount of protein digested compared to the amount of peptide recovered for LC-IMS-MS/MS analysis (B)

    Results

    Supplemental Figure 3 - Linear regression analysis of protein abundances measured using myoglobin as a standard...

    Supplemental Table 2 - Appraisal of the protein standards used for the label-free quantitative analysis

    Supplemental Table 3 - The precision (%CV) of label-free measurements, their relative error (%RE) compared to QconCAT measurements and differences between the two sets of measurements

    Supplemental Figure 4 - Venn diagrams showing the overlap between all quantified human liver microsomal proteins...

    Supplemental Figure 5 - Venn diagrams of the subcellular localization of all proteins...

    Supplemental Figure 6 - Results of ion mobility spectrometry (IMS) in sample HLM01 illustrating effective separation of peptide ions in the mobility domain...

    References

  View article

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