Comparison of methods for estimating unbound intracellular-to-medium concentration ratios in rat and human hepatocytes using statins

Data Supplement

• Supplemental Data -

  Supplemental Text - Theoretical derivation of the equations for Kp,uu considering the
  plasma membrane potential of cells and the fraction of drugs ionized
  

  Supplemental Table 1 - Intracellular volumes of rat and human hepatocytes
  

  Supplemental Figure 1 - The pH-dependent membrane permeation clearance of pitavastatin (A),
  rosuvastatin (B), and pravastatin (C) across Caco-2 cell monolayers was measured in an apical-tobasal
  direction with an apical pH of 5.5, 6.0, 6.5, and 7.4, and a basal pH of 7.4 in the presence of
  rifamycin SV (100 mu-M), cyclosporin A (10 mu-M), and Ko143 (10 mu-M)
  

  Supplemental Figure 2 - Concentration-dependent uptake of pitavastatin (A), rosuvastatin (B), and
  pravastatin (C) by rat hepatocytes
  

  Supplemental Figure 3 - Concentration-dependent uptake of pitavastatin (A, D), rosuvastatin (B, E),
  and pravastatin (C) by human hepatocytes