Data Supplement
- Supplemental Data -
Supplemental Methods
Supplemental Figure 1 - Compound AZD1979 from (Johansson et al., 2016; Li et al., 2016)
Supplemental Figure 2 - Effect of P450 inhibitors ketoconazole (KTZ; 200 μM) and 1-
aminobenzotriazole (1-ABT; 2 mM), the mEH inhibitor valpromide (VPD) and the sEH inhibitor
t-AUCB (20 μM) on the hydration of compounds 2-9, 15-20, cis-stilbene oxide (cSO; mEH probe
substrate) and 11,12-EET (sEH probe substrate)Supplemental Figure 3 - Kinetic constant determination for the metabolism of compounds 4-7,
15 and 18 by human recombinant mEHSupplemental Figure 4 - Kinetic constant determination for the metabolism of compounds 4-7,
15 and 18 by HLMSupplemental Figure 5 - Synthetic diol standards used for identification of diol metabolites
formed in enzyme assays with liver fractions and for quantification of metabolism by
recombinant mEHSupplemental Table 1 - UHPLC-QTOF-MS and MSE Data for Compounds 1-9 and their Diol
Metabolites Formed in Incubations with HLM and NADPHSupplemental Table 2 - Ring strain energy estimates of relevant truncated fragments of
compounds 1-9, AZD1979 and the mEH probe substrates cis-stilbene oxide and styrene 7,8-
oxideSupplemental Figure 6 - 1H NMR spectra of compounds 1-22
Supporting references