Direct Quantification of Cytochromes P450 and Drug Transporters - A Rapid, Targeted Mass Spectrometry-Based Immunoassay Panel for Tissues and Cell Culture Lysates

Data Supplement

• Supplemental Data -

  Supplemental Figure 1 - Binding motifs of multi-specific TXP-antibodies

  Supplemental Figure 2 - Working range of 24 MS-based immunoassays

  Supplemental Figure 3 - Accuracy and recovery of 24 MS-based immunoassays

  Supplemental Figure 4 - Induction studies. Induction qualified human cryopreserved hepatocytes were cultivated in
  96-well plates

  Supplemental Table 1 - Correlation factors calculated from quantification results generated by measuring CYP
  enzyme and transporter concentrations from liver tissue (T) (set A), crude membrane/nuclei fraction
  (CMF) and microsomal fraction (M). Mean, CV and Pearson correlation factor are given

  Supplemental Table 2 - Quantification of CYP enzymes and transporters (technical replicates, n=3) in liver tissue (T)
  (set A), crude membrane/nuclei fraction (CMF), microsomal fraction (M), and cytosol (C) of ten liver
  samples

  Supplemental Table 3 - Interday reproducibility and variation of MS-based immunoassays of 15 human liver tissues
  (set B)