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Research ArticleArticle

Recellularized native kidney scaffolds as a novel tool in nephrotoxicity screening

Michele Fedecostante, Koen Westphal, Michele Buono, Natalia Sanchez Romero, Martijn J Wilmer, Janis Kerkering, Pedro Miguel Baptista, Joost G Hoenderop and Rosalinde Masereeuw
Drug Metabolism and Disposition July 6, 2018, dmd.118.080721; DOI: https://doi.org/10.1124/dmd.118.080721
Michele Fedecostante
1 Utrecht Institute for Pharmaceutical Sciences;
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Koen Westphal
1 Utrecht Institute for Pharmaceutical Sciences;
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Michele Buono
1 Utrecht Institute for Pharmaceutical Sciences;
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Natalia Sanchez Romero
2 Aragon Health Research Institute;
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Martijn J Wilmer
3 Radboud Institute for Molecular Life Sciences;
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Janis Kerkering
3 Radboud Institute for Molecular Life Sciences;
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Pedro Miguel Baptista
4 Aragon Health research Institute
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Joost G Hoenderop
3 Radboud Institute for Molecular Life Sciences;
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Rosalinde Masereeuw
1 Utrecht Institute for Pharmaceutical Sciences;
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  • ORCID record for Rosalinde Masereeuw
  • For correspondence: r.masereeuw@uu.nl

Data Supplement

  • Supplemental Data -

    Supplemental Table 1 - Taqman® primer-probe sets list

    Supplemental Figure 1 - ZO-1 and CLD-2 Immunostainings of ciPTEC 2D cultures

    Supplemental Figure 2 - Live/Dead assay on cisplatin-, tenofovir- and cyclosporine-treated ciPTECOAT1 2D and 3D cultures

    Supplemental Figure 3 - GAPDH and HPRT1 Cycle threshold values in ciPTEC 2-D and RS

    Supplemental Figure 4 - GAPDH and HPRT1 Cycle threshold values in ciPTEC 2-D and RS

    Supplemental Figure 5 - ASP+ uptake with live imaging

    Supplemental Figure 6 - Fluorescein uptake with live imaging

    Supplemental Figure 7 - Calcein uptake with live imaging

    Supplemental Figure 8 - Navitoclax nephrotoxicity assay in recellularized scaffolds and ciPTEC-OAT1 2-D cultures

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