Data Supplement
- Supplemental Data -
Supplemental Figure 1 - Different strains of F. prausnitzii (i.e., A2-165, ATCC 27766 and ATCC 27768) were cultured overnight in YCFA media, followed by anaerobic incubation with tacrolimus (100 μg/ml) at 37°C for 48 h
Supplemental Figure 2 - MS2 spectra of tacrolimus and M1 (IT-TOF)
Supplemental Figure 3 - MS2 fragments of tacrolimus and M1 obtained in IT-TOF and QTOF systems
Supplemental Figure 4 - Tacrolimus (50 mg) was incubated with F. prausnitzii A2-165 (OD ~2; 500 ml) for 96 h, followed by ethyl acetate extraction of the mixture
Supplemental Figure 5 - IR spectra of a) tacrolimus, b) M1, c) overlay of tacrolimus (red) and M1 (blue) spectra
Supplemental Figure 6 - 1H NMR spectrum (900 MHz, CDCl3) of M1
Supplemental Figure 7 - DEPTQ spectrum (226 MHz, CDCl3) of M1
Supplemental Figure 8 - COSY spectrum (900 MHz, CDCl3) of M1
Supplemental Figure 9 - HSQC spectrum (900 MHz, CDCl3) of M1
Supplemental Figure 10 - HMBC spectrum (900 MHz, CDCl3) of M1
Supplemental Figure 11 - 1H-13C HSQC-TOCSY spectrum (900 MHz, CDCl3, 90 ms mixing time) of M1
Supplemental Figure 12 - Key 2D NMR correlations of M1 (Isomer I)
Supplemental Figure 13 - Expansions of the DEPTQ spectra (226 MHz, CDCl3) of M1 (three major isomers assigned) and tacrolimus (two isomers)
Supplemental Figure 14 - Bacteria cultured overnight in YCFA media were incubated with tacrolimus (100μg/ml) or vehicle control anaerobically at 37°C for 48 h
Supplemental Figure 15 - Tacrolimus (10 μg/ml) was incubated with F. prausnitzii (6.28×107 CFU/ml) anaerobically at 37°C up to 24 h
Supplemental Table 1 - List of commensal gut bacteria obtained from Biodefense and Emerging Infections (BEI) Research Resources Repository
Supplemental Table 2 - Demographic information of kidney transplant patients whose stool samples were analyzed
Supplemental Table 3 - NMR spectroscopic data of M1 (Isomer I), CDCl3a
Supplemental Table 4 - NMR Spectroscopic Data of M1 (Isomer II), CDCl3a