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OtherArticle

Functional proliferating human hepatocytes: in vitro hepatocyte model for drug metabolism, excretion and toxicity

Shida Qiao, Sisi Feng, Zhitao Wu, Ting He, Chen Ma, Zhaoliang Peng, E Tian and Guoyu Pan
Drug Metabolism and Disposition February 1, 2021, DMD-AR-2020-000275; DOI: https://doi.org/10.1124/dmd.120.000275
Shida Qiao
1Shanghai Institute of Materia Medica, China
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Sisi Feng
2Shanghai Hexaell Biotech Co., Ltd, China
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Zhitao Wu
3Shanghai Institute of Materia Medica; Nanjing University of Chinese Medicine, China
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Ting He
4Nanjing Tech University, China
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Chen Ma
1Shanghai Institute of Materia Medica, China
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Zhaoliang Peng
1Shanghai Institute of Materia Medica, China
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E Tian
2Shanghai Hexaell Biotech Co., Ltd, China
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  • For correspondence: gypan@simm.ac.cn
Guoyu Pan
1Shanghai Institute of Materia Medica, China
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  • For correspondence: gypan@simm.ac.cn

Data Supplement

  • Data Supplement -

    Supplemental Table 1 - Demographics, supplier and metabolic activity of primary human hepatocytes used for metabolism, disposition and toxicity. 

    Supplemental Table 2 - HPLC/MS-MS conditions and parameters for CYP450 enzyme activity assays.

    Supplemental Table 3 - Primer sequences used for RT-qPCR.

    Supplemental Table 4 - In vitro toxicity test TC50 values in PHH, ProliHH-P and ProliHH-M for 12 compounds.

    Supplemental Figure 1 - Morphology of ProliHH at indicated days in HM culture.

    Supplemental Figure 2 - ProliHH were proliferative in HM culture.

    Supplemental Figure 3 - Impact of culture time on CYP450 mRNA expression in ProliHH.

    Supplemental Figure 4 - Protein expression level of CYP2B6 enzyme in ProliH-M.

    Supplemental Figure 5 - Effect of cholestatic drugs on d8-TCA efflux in sandwich cultured ProliHH.

    References

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