Functional proliferating human hepatocytes: in vitro hepatocyte model for drug metabolism, excretion and toxicity

Data Supplement

• Data Supplement -

  Supplemental Table 1 - Demographics, supplier and metabolic activity of primary human hepatocytes used for metabolism, disposition and toxicity. 

  Supplemental Table 2 - HPLC/MS-MS conditions and parameters for CYP450 enzyme activity assays.

  Supplemental Table 3 - Primer sequences used for RT-qPCR.

  Supplemental Table 4 - In vitro toxicity test TC₅₀ values in PHH, ProliHH-P and ProliHH-M for 12 compounds.

  Supplemental Figure 1 - Morphology of ProliHH at indicated days in HM culture.

  Supplemental Figure 2 - ProliHH were proliferative in HM culture.

  Supplemental Figure 3 - Impact of culture time on CYP450 mRNA expression in ProliHH.

  Supplemental Figure 4 - Protein expression level of CYP2B6 enzyme in ProliH-M.

  Supplemental Figure 5 - Effect of cholestatic drugs on d8-TCA efflux in sandwich cultured ProliHH.

  References