Quantification of accurate composition and total abundance of homologous proteins by conserved-plus-surrogate peptide (CPSP) approach: Quantification of UDP glucuronosyltransferases in human tissues

Data Supplement

• Supplemental Data -

  Supplemental Table 1. List of conserved and surrogate peptides used for targeted LC-MS/MS quantification of UGT proteins.

  Supplemental Table 2. Chromatographic conditions to separate UGT surrogate and conserved peptides. 

  Supplemental Table 3.  UGTs abundance (pmol/mg microsomal protein) in HLM, HIM, HKM, and HLS9. 

  Supplemental Figure 1. Representative chromatogram of two conserved peptides, IPQTVLWR (A) and VLWR (B) used in the quantification of UGT1A and UGT2B enzymes, respectively.

  Supplemental Figure 2. The calibration curves of the conserved peptides, IPQTVLWR (A) and VLWR, (B) were linear between 58 to 925 and 0.33 to 170.75 fmol on-column, respectively with R2 >0.98.

  Supplemental Figure 3. The abundance of UGT1As (A) and UGT2Bs (B) using the conserved peptide approach in the HLS9 fractions.