PT  - JOURNAL ARTICLE
AU  - H J Nelis
AU  - A P De Leenheer
TI  - Metabolism of minocycline in humans.
DP  - 1982 Mar 01
TA  - Drug Metabolism and Disposition
PG  - 142--146
VI  - 10
IP  - 2
4099  - http://dmd.aspetjournals.org/content/10/2/142.short
4100  - http://dmd.aspetjournals.org/content/10/2/142.full
SO  - Drug Metab Dispos1982 Mar 01; 10
AB  - Metabolites of tetracycline antibiotics have not previously been isolated from human excreta. It is now demonstrated that minocycline is the first tetracycline to be metabolized in man. A two-step liquid chromatographic procedure was used to isolate three metabolites from human urine. The principal metabolite was tentatively identified as 9-hydroxyminocycline by mass spectral and spectrophotometric analysis and comparison with a synthetic compound. The latter was prepared by incubation of minocycline in a modified Udenfriend system, simulating an enzymatic oxygenase. Two other major metabolites are probably mono-N-demethylated derivatives. Substantial amounts of 4-epiminocycline, which probably resulted from minocycline epimerization rather than biotransformation, were also present.