@article {Maguire595, author = {J H Maguire and T C Butler and K H Dudley}, title = {5-ethyl-5-phenylhydantoin N-glucuronide, the major urinary metabolite of 5-ethyl-5-phenylhydantoin (Nirvanol) in the dog.}, volume = {10}, number = {6}, pages = {595--598}, year = {1982}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {A water-soluble metabolite, isolated from the urine of dogs given (S)-5-ethyl-5-phenylhydantoin [(S)-EPH], has been identified as 1-deoxy-1-[(5S)-5-ethyl-5-phenylhydantoin-3-yl] beta-D-glucopyranuronate [(S)-EPH N-glucuronide]. EPH N-glucuronide did not release the aglycone upon acid or beta-glucuronidase treatment, but incubation in alkaline solution (pH 12-13) readily formed 2-ethyl-2-phenylhydantoic acid (EPHA). The EPHA so formed could be quantitatively cyclized to EPH. With the knowledge of the conversion efficiency of EPH N-glucuronide to EPHA, a quantitative GLC assay for the metabolite was developed. EPH N-glucuronide was found to be the major urinary metabolite after administration to dogs of either (R)-, (S)-, or (RS)-EPH.}, issn = {0090-9556}, URL = {https://dmd.aspetjournals.org/content/10/6/595}, eprint = {https://dmd.aspetjournals.org/content/10/6/595.full.pdf}, journal = {Drug Metabolism and Disposition} }