@article {Miller527, author = {M J Miller and I G Sipes and D F Perry and D E Carter}, title = {Pharmacokinetics of o-nitroanisole in Fischer 344 rats.}, volume = {13}, number = {5}, pages = {527--531}, year = {1985}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {The pharmacokinetics and metabolism of o-nitroanisole (ONA) were studied in male Fischer 344 rats. Three dose levels of [14C]ONA (5.0, 50, or 500 mg/kg) were administered orally to rats and daily excreta were analyzed for 14C. Since the highest dose altered the urinary excretion rate of ONA, a dose of 25 mg/kg was used for subsequent pharmacokinetic studies. Following a single 25 mg/kg iv dose of [14C]ONA, blood, tissues, and excreta were collected at times ranging from 15 min to 7 days. Urinary excretion accounted for 82\% of the dose by 24 hr and 86\% by 7 days. Fecal excretion was 7.5\% in 24 hr and 9.0\% by 7 days. Fifteen min after ONA administration, most of the 14C content was found in muscle (20\%), skin (10\%), adipose tissue (6.8\%), and blood (6.5\%). All other tissues contained less than 5\% of the dose. Within 8 hr, less than 1\% of the dose was present in any tissue. The initial elimination t1/2 for 14C in all tissues was 1-2 hr and the terminal t1/2 was approximately 4 days. The elimination of parent ONA from blood followed first order biphasic elimination kinetics (initial t1/2 = 30 min; terminal t1/2 = 2.2 hr). Parent ONA was rapidly eliminated from all other tissues in a monophasic manner (t1/2 = 15 min to 2 hr). Skin and fat demonstrated an uptake phase prior to the elimination of parent. Only 0.5\% of the dose was excreted as ONA in the urine. Urinary metabolites of ONA were predominantly conjugated compounds (63\% as sulfates; 11\% as glucuronides).}, issn = {0090-9556}, URL = {https://dmd.aspetjournals.org/content/13/5/527}, eprint = {https://dmd.aspetjournals.org/content/13/5/527.full.pdf}, journal = {Drug Metabolism and Disposition} }