@article {Knupp816, author = {C A Knupp and D G Corley and M S Tempesta and S P Swanson}, title = {Isolation and characterization of 4{\textquoteright}-hydroxy T-2 toxin, a new metabolite of the trichothecene mycotoxin T-2.}, volume = {15}, number = {6}, pages = {816--820}, year = {1987}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {Biotransformation of the trichothecene mycotoxin T-2 by the hepatic S-9 fraction prepared from phenobarbital-treated rats yielded a new metabolic product designated RLM-3. The metabolite was purified from the hepatic preparation using preparative HPLC. The structural analysis of RLM-3 was carried out using gas chromatography/mass spectrometry and 1H and 13C NMR. RLM-3 was identified as 4{\textquoteright}-hydroxy T-2. The toxicity of RLM-3 in comparison to T-2 toxin and 3{\textquoteright}-hydroxy T-2 was assessed using a rat skin bioassay technique. The metabolite 4{\textquoteright}-hydroxy T-2 was shown to be deacylated at the C-4 position to yield 4{\textquoteright}-hydroxy HT-2 when incubated with rat hepatic S-9 preparations.}, issn = {0090-9556}, URL = {https://dmd.aspetjournals.org/content/15/6/816}, eprint = {https://dmd.aspetjournals.org/content/15/6/816.full.pdf}, journal = {Drug Metabolism and Disposition} }