@article {Inaba605, author = {T Inaba and A Tait and M Nakano and W A Mahon and W Kalow}, title = {Metabolism of diazepam in vitro by human liver. Independent variability of N-demethylation and C3-hydroxylation.}, volume = {16}, number = {4}, pages = {605--608}, year = {1988}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {The interindividual variability of diazepam metabolism was studied using human livers. The formation of N-desmethyldiazepam (NDZ) and 3-hydroxydiazepam (temazepam, TMZ), was monitored by gas chromatography. In 10 livers, Km values for NDZ and TMZ formation varied independently from each other, each by a factor of 4, from 100 to 400 microM. This variability is consistent with the presence of at least two different cytochrome P-450 species controlling formation of these metabolites. In the same livers, Vmax for NDZ and TMZ production varied 6-fold and 15-fold, respectively.}, issn = {0090-9556}, URL = {https://dmd.aspetjournals.org/content/16/4/605}, eprint = {https://dmd.aspetjournals.org/content/16/4/605.full.pdf}, journal = {Drug Metabolism and Disposition} }