TY - JOUR T1 - The use of pig hepatocytes to study the biotransformation of beta-nortestosterone by pigs. Identification of glucuronidated 15 alpha-hydroxy-norandrostenedione as an important in vitro and in vivo metabolite. JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 999 LP - 1004 VL - 18 IS - 6 AU - L A Hoogenboom AU - M C Berghmans AU - A Van Veldhuizen AU - H A Kuiper Y1 - 1990/11/01 UR - http://dmd.aspetjournals.org/content/18/6/999.abstract N2 - Porcine hepatocytes were used to examine the biotransformation of beta-nortestosterone (NOR). Initially, the major metabolite of NOR was norandrostenedione (NA), which upon prolonged incubation was further transformed, primarily to the glucuronide of 15 alpha-hydroxy-norandrostenedione (15 alpha OH-NA). No differences were observed in this pattern between hepatocytes isolated from livers of sows or castrated male pigs. With increasing culture age, the rate of formation of NA did not change, contrary to a decrease in its further oxidation and glucuronidation. 15 alpha-OH-NA, primarily as its glucuronide, was identified in the urine of pigs injected with NOR. In addition, NA and the glucuronide of the parent compound were present in much smaller amounts. ER -