@article {Hezari882, author = {M Hezari and P J Davis}, title = {Microbial models of mammalian metabolism. N-dealkylation of furosemide to yield the mammalian metabolite CSA using Cunninghamella elegans.}, volume = {20}, number = {6}, pages = {882--888}, year = {1992}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {Furosemide (Lasix), a widely used diuretic, is metabolized by the fungus Cunninghamella elegans (ATCC 36112) to 4-chloro-5-sulfamoyl anthranilic acid (CSA), a metabolite also present in mammalian systems. This metabolite was isolated following preparative-scale incubations of C. elegans, and was characterized by comparison with standard CSA using 13C-NMR, mass spectrometry (high-resolution mass spectra, electron impact mass spectra), UV, TLC, and HPLC with fluorescence detection. Because a known complication with furosemide studies is the spontaneous formation of CSA by decomposition of furosemide during incubation, extraction, and/or analysis, a time course study was conducted to determine the rate of CSA formation caused by metabolism vs. the relatively low rate of CSA formation caused by spontaneous decomposition.}, issn = {0090-9556}, URL = {https://dmd.aspetjournals.org/content/20/6/882}, eprint = {https://dmd.aspetjournals.org/content/20/6/882.full.pdf}, journal = {Drug Metabolism and Disposition} }