PT  - JOURNAL ARTICLE
AU  - M M Vestling
AU  - C M Murphy
AU  - D A Keller
AU  - C Fenselau
AU  - J Dedinas
AU  - D L Ladd
AU  - M A Olsen
TI  - A strategy for characterization of polyethylene glycol-derivatized proteins. A mass spectrometric analysis of the attachment sites in polyethylene glycol-derivatized superoxide dismutase.
DP  - 1993 Sep 01
TA  - Drug Metabolism and Disposition
PG  - 911--917
VI  - 21
IP  - 5
4099  - http://dmd.aspetjournals.org/content/21/5/911.short
4100  - http://dmd.aspetjournals.org/content/21/5/911.full
SO  - Drug Metab Dispos1993 Sep 01; 21
AB  - Base treatment of polyethylene glycol-derivatized superoxide dismutase in which the polyethylene glycol is linked to the protein via a succinyl bridge, removes the polyethylene glycol leaving a succinyl marker. Exhaustive succinylation with d4-succinic anhydride completes the derivatization in order to minimize fractionation in proteolysis, chromatography, and desorption in the mass spectrometer. Production of peptides from the derivatized protein for high resolution and high-resolution tandem MS allows identification of the site that had been derivatized by polyethylene glycol and the determination of the amount of polyethylene glycol originally at each site. The mass spectrometric strategy outlined herein can be applied to other proteins derivatized for therapeutic administration.