TY - JOUR T1 - HEPATIC MICROSOMAL MIXED-FUNCTION OXIDASE ACTIVITY OF SEVERAL MARINE SPECIES FROM COASTAL MAINE JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 545 LP - 555 VL - 2 IS - 6 AU - ROBERTA J. POHL AU - JOHN R. BEND AU - ANTHONY M. GUARINO AU - JAMES R. FOUTS Y1 - 1974/11/01 UR - http://dmd.aspetjournals.org/content/2/6/545.abstract N2 - Mixed-function oxidase activity toward aniline·HCl, d-benzphetamine·HCl, 7-ethoxycoumarin, and benzpyrene of microsomes from various tissues of crustacea, cyclostomes, elasmobranchs, and teleosts was studied. 1) Negligible activity was detectable in rock crab or lobster hepatopancreas microsomes, possibly because of inhibition by digestive juice. 2) Activity per mg of hepatic microsomal protein in the other species was about 1/10 that seen in rat or rabbit liver microsomes, although cytochrome P-450 levels were about 25% of those seen in rat or rabbit liver microsomes. 3) Dogfish hepatic microsomal 7-ethoxycoumarin deethylase began to be inactivated at temperatures greater than 30-32°C, whereas mouse and rabbit enzymes showed no evidence of inactivation at temperatures up to 45°C. 4) Winter flounder and mummichog hepatic microsomal benzpyrene hydroxylase activities were 10 times higher than those assayed in the other species; winter flounder hepatic microsomal benzpyrene hydroxylase was not as inducible as that in little skate liver. 5) Kidney was the only extra-hepatic tissue found to contain more than barely detectable microsomal mixed-function oxidase activity in the species studied. Copyright © 1974 by The American Society for Pharmacology and Experimental Therapeutics ER -