TY - JOUR T1 - A Fluorescent Assay Amenable to Measuring Production of β-<span class="sc">d</span>-Glucuronides Produced from Recombinant UDP-Glycosyl Transferase Enzymes JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 555 LP - 557 VL - 27 IS - 5 AU - Olga V. Trubetskoy AU - Peter M. Shaw Y1 - 1999/05/01 UR - http://dmd.aspetjournals.org/content/27/5/555.abstract N2 - β-glucuronidase cleavage of 4-methylumbelliferyl β-d-glucuronide generates the highly fluorescent compound, 4-methylumbelliferone. We show that other β-d-glucuronide compounds act as competitors in this assay. The 4-methylumbelliferyl β-d-glucuronide cleavage assay can easily be adapted to high throughput formats to detect the presence of β-D glucuronides generated using recombinant glycosyl transferase preparations. The American Society for Pharmacology and Experimental Therapeutics ER -