RT Journal Article
SR Electronic
T1 Reductive Metabolism In Vivo of<em>Trans-</em>4-Phenyl-3-buten-2-one in Rats and Dogs
JF Drug Metabolism and Disposition
JO Drug Metab Dispos
FD American Society for Pharmacology and Experimental Therapeutics
SP 767
OP 769
VO 27
IS 7
A1 Shigeyuki Kitamura
A1 Yuji Okamoto
A1 Mitsuhiro Takeshita
A1 Shigeru Ohta
YR 1999
UL http://dmd.aspetjournals.org/content/27/7/767.abstract
AB The reductive metabolism in vivo of a flavoring additive,trans-4-phenyl-3-buten-2-one (PBO;trans-methyl styryl ketone) was investigated in rats and dogs. In both species, the double bond-reduced product, 4-phenyl-2-butanone (PBA), was detected by HPLC as the predominant species in blood after i.v. administration of PBO. PBA detected in rat blood was identified by comparison to the authentic sample. In contrast, the carbonyl-reduced product,trans-4-phenyl-3-buten-2-ol (PBOL) was also detected as a minor metabolite of PBO in both species. The area under the curve of PBOL in rat blood was only 3% of that of PBA. PBO was mutagenic in the Ames test using Salmonella typhimurium TA 100 when S-9 mix was added, but PBA and PBOL were not. It appears that PBO is mainly metabolized to PBA in vivo in rats and dogs as a detoxification pathway. The American Society for Pharmacology and Experimental Therapeutics