TY - JOUR T1 - Ethynylestradiol-Mediated Induction of Hepatic<em>CYP3A9</em> in Female Rats: Implication for Cyclosporine Metabolism JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 1505 LP - 1511 VL - 27 IS - 12 AU - Walter Jäger AU - Maria Almira Correia AU - Lester M. Bornheim AU - Axel Mahnke AU - Walter G. Hanstein AU - Linlong Xue AU - Leslie Z. Benet Y1 - 1999/12/01 UR - http://dmd.aspetjournals.org/content/27/12/1505.abstract N2 - Repeated treatment of female rats with the synthetic estrogen ethynylestradiol (EE2) increases the formation of the cyclosporine A (CyA) metabolites AM1c and AM9 by 3-fold, whereas the formation of AM1 and AM4N is not significantly enhanced. The formation of all four CyA metabolites was inhibited by greater than 80% by the CYP3A-selective substrate midazolam or polyclonal anti-rat CYP3A IgGs in liver microsomes of untreated and EE2-induced rats. In contrast, anti-rat CYP2C6 IgGs had little effect, indicating the involvement of a CYP3A but not 2C6 in this EE2-stimulated CyA metabolism. Semiquantitative reverse-transcriptase polymerase chain reaction was used to determine the mRNA content for four CYP3A genes (CYP3A2, CYP3A9, CYP3A18, and CYP3A23) in livers of control and EE2-treated female rats. EE2 selectively induced CYP3A9 by 3.3-fold whereas the expression of CYP3A18 and CYP3A23 was slightly decreased; neither CYP3A2 mRNA nor CYP3A1 mRNA was detectable in these EE2-treated livers. To determine whether rat liver microsomal CYP3A9 was indeed responsible for the EE2-stimulated CyA metabolism, a recombinant CYP3A9 was heterologously expressed in Escherichia coli. When functionally reconstituted, this enzyme was active in metabolizing CyA preferentially to its AM9 and AM1c metabolites as compared with CYP3A4. These findings thus support the notion that the increased CyA-metabolizing capacity of EE2-treated female rat liver microsomes is due to the induction of the CYP3A9 enzyme. The American Society for Pharmacology and Experimental Therapeutics ER -