PT  - JOURNAL ARTICLE
AU  - Linh T. Nguyen
AU  - Murali Ramanathan
AU  - Bianca Weinstock-Guttman
AU  - Kiran Dole
AU  - Colleen Miller
AU  - Margaret Planter
AU  - Kara Patrick
AU  - Carol Brownscheidle
AU  - Lawrence D. Jacobs
TI  - Detection of Cytochrome P450 and Other Drug-Metabolizing Enzyme mRNAs in Peripheral Blood Mononuclear Cells Using DNA Arrays
DP  - 2000 Aug 01
TA  - Drug Metabolism and Disposition
PG  - 987--993
VI  - 28
IP  - 8
4099  - http://dmd.aspetjournals.org/content/28/8/987.short
4100  - http://dmd.aspetjournals.org/content/28/8/987.full
SO  - Drug Metab Dispos2000 Aug 01; 28
AB  - The purpose of this study was to determine whether the expression of cytochrome P450 (CYP) enzyme mRNAs, other drug-metabolizing enzyme mRNAs, and transporter mRNAs can be detected using DNA arrays. Total RNA was isolated from peripheral blood mononuclear cells of 10 multiple sclerosis patients and 10 age- and sex-matched controls. The mRNA was reverse transcribed to radiolabeled cDNA, and the resultant cDNA was used to probe a DNA array containing several thousand known human genes. The signals corresponding to several CYPs, drug-metabolizing, and transporter mRNAs was substantially above background. The results demonstrate that the DNA array technique has the sensitivity and the selectivity for applications in the pharmaceutical sciences. The mean values for mRNAs of specific CYPs and drug-metabolizing enzymes in peripheral blood cells were compared with reported values for liver. The capabilities of DNA arrays may prove useful for characterizing CYP expression in a variety of clinical samples. The American Society for Pharmacology and Experimental Therapeutics