RT Journal Article SR Electronic T1 Application of Directly Coupled High Performance Liquid Chromatography-NMR-Mass Spectometry and 1H NMR Spectroscopic Studies to the Investigation of 2,3-Benzofuran Metabolism in Sprague-Dawley Rats JF Drug Metabolism and Disposition JO Drug Metab Dispos FD American Society for Pharmacology and Experimental Therapeutics SP 1357 OP 1363 DO 10.1124/dmd.30.12.1357 VO 30 IS 12 A1 John C. Connelly A1 Susan C. Connor A1 Soria Monte A1 Nigel J.C. Bailey A1 Nathan Borgeaud A1 Elaine Holmes A1 Jeff Troke A1 Jeremy K. Nicholson A1 Claire L. Gavaghan YR 2002 UL http://dmd.aspetjournals.org/content/30/12/1357.abstract AB The urinary excretion of metabolites of 2,3-benzofuran was studied in Sprague-Dawley rats (n = 5) given a single dose of 150 mg/kg i.p. Urine samples were collected at defined intervals up to 7 days postdose and analyzed using 1H NMR and directly coupled high performance liquid chromatography (HPLC)-NMR, HPLC-(mass spectrometry) MS and HPLC-MS-NMR methods. The principal metabolites were determined to be 2-hydroxyphenylacetic acid and 2-(2-hydroxyethyl)phenyl hydrogen sulfate, representing 24.3 ± 6.0% and 19.6 ± 6.4% of the dose, respectively. This indicates that metabolism of benzofuran to the polar species excreted in urine involves cleavage of the furan ring. The American Society for Pharmacology and Experimental Therapeutics