RT Journal Article
SR Electronic
T1 Real-Time Quantitative Polymerase Chain Reaction for MDR1, MRP1, MRP2, and CYP3A-mRNA Levels in Caco-2 Cell Lines, Human Duodenal Enterocytes, Normal Colorectal Tissues, and Colorectal Adenocarcinomas
JF Drug Metabolism and Disposition
JO Drug Metab Dispos
FD American Society for Pharmacology and Experimental Therapeutics
SP 4
OP 6
DO 10.1124/dmd.30.1.4
VO 30
IS 1
A1 Tsutomu Nakamura
A1 Toshiyuki Sakaeda
A1 Nobuko Ohmoto
A1 Takao Tamura
A1 Nobuo Aoyama
A1 Toshiro Shirakawa
A1 Takashi Kamigaki
A1 Takeshi Nakamura
A1 Ke Ih Kim
A1 Soo Ryang Kim
A1 Yoshikazu Kuroda
A1 Masafumi Matsuo
A1 Masato Kasuga
A1 Katsuhiko Okumura
YR 2002
UL http://dmd.aspetjournals.org/content/30/1/4.abstract
AB The expression levels of mRNAs for MDR1 (P-glycoprotein), multidrug resistance-associated proteins (MRP1, MRP2), and cytochrome P450 3A (CYP3A) in Caco-2 cells were quantitatively compared with those in human duodenal enterocytes, normal colorectal tissues, and colorectal adenocarcinomas. Caco-2 cells (passages 36–88) were kindly supplied by several laboratories in Japan. Human duodenal enterocytes were obtained from five healthy male volunteers. Normal colorectal tissues and colorectal adenocarcinomas were simultaneously obtained from seven patients with primary colorectal adenocarcinoma. MDR1, MRP1, MRP2, and CYP3A mRNA levels were determined by real-time quantitative polymerase chain reactions (PCR). Relative concentrations of mRNAs for target proteins (MDR1, MRP1, MRP2, and CYP3A) and glyceraldehyde-3-phosphate dehydrogenase in Caco-2 cells were 1.00 ± 0.15, 1.02 ± 0.06, 0.94 ± 0.10, and 0.68 ±0.60, respectively, and those in human enterocytes were about 12-, 3-, 7-, and 8000-fold higher than in the Caco-2 cells, respectively. In contrast, MDR1, MRP1, and CYP3A mRNA levels in Caco-2 cells were comparable to those in normal colorectal tissue and colorectal adenocarcinoma. The American Society for Pharmacology and Experimental Therapeutics