PT  - JOURNAL ARTICLE
AU  - Liyue Huang
AU  - Mary Vore
TI  - Multidrug Resistance P-Glycoprotein 2 Is Essential for the Biliary Excretion of Indocyanine Green
DP  - 2001 May 01
TA  - Drug Metabolism and Disposition
PG  - 634--637
VI  - 29
IP  - 5
4099  - http://dmd.aspetjournals.org/content/29/5/634.short
4100  - http://dmd.aspetjournals.org/content/29/5/634.full
SO  - Drug Metab Dispos2001 May 01; 29
AB  - Multidrug resistance P-glycoprotein 2 (Mdr2) is a phospholipid translocator in the canalicular membrane that is essential for the formation of biliary phospholipid vesicles and mixed lipid/bile salt micelles. Incorporation into biliary vesicles and micelles is thought to contribute to the hepatobiliary excretion of certain hydrophobic organic anions, such as indocyanine green (ICG). The present studies characterized the biliary excretion of two hydrophobic organic anions, ICG and estradiol-17β(β-d-glucuronide) (E217G), in the single-pass isolated perfused liver and the biliary excretion of glutathione (GSH) in vivo in wild-type andMdr2−/− female mice. The biliary excretion of ICG (0.4 μmol) was reduced by 90%, while the biliary excretion of total GSH was decreased by 65% in Mdr2−/−mice relative to wild-type mice. In contrast, the biliary excretion of E217G (0.1 μmol) was increased by 30% inMdr2−/− mice. These data indicate that the absence of Mdr2 differentially influences the biliary excretion of these organic anions and suggest that phospholipid vesicles and mixed micelles in bile are essential for the biliary excretion of ICG. The American Society for Pharmacology and Experimental Therapeutics