RT Journal Article SR Electronic T1 METABOLISM OF 1-(o-CHLOROPHENYL)-1-(p-CHLOROPHENYL)-2,2-DICHLOROETHANE (o,p’-DDD) IN RATS JF Drug Metabolism and Disposition JO Drug Metab Dispos FD American Society for Pharmacology and Experimental Therapeutics SP 15 OP 25 VO 3 IS 1 A1 V. D. REIF A1 J. E. SINSHEIMER YR 1975 UL http://dmd.aspetjournals.org/content/3/1/15.abstract AB The metabolism of 14C-labeled 1-(o-chlorophenyl)-1-(p-chlorophenyl)-2,2-dichloroethane (o,p’-DDD) in rats was investigated. Metabolites were identified in feces and urine extracts by thin-layer chromatography (TLC), gas-liquid chromatography, and mass spectrometry. Extracts of acidic metabolites were methylated with diazomethane for identification. Metabolites were quantitated by TLC and liquid scintillation counting. After a 100-mg oral dose to each of three rats, an average of 7.1% of the radioactivity was excreted in the urine and 87.8% in the feces within 8 days. The urine was found to contain o,p’-dichlorodiphenylacetic acid (o,p’-DDA) as well as 4-hydroxy-, 3-hydroxy-, and 3,4-dihydroxy-substituted o,p’-DDA. The serine and glycine conjugates of o,p’-DDA were also identified. In addition to the above metabolites the feces contained o,p’-DDD, 1-(o-chlorophenyl)-1-(p-chlorophenyl)-2-chloroethylene, and the aspartic acid conjugate of o,p’-DDA. The presence of aromatic mono- and dihydroxylated o,p’-DDD was also detected in feces. Copyright © 1975 by The American Society for Pharmacology and Experimental Therapeutics