TY - JOUR T1 - ENANTIOSELECTIVITY OF HUMAN HYDROXYSTEROID SULFOTRANSFERASE ST2A3 WITH NAPHTHYL-1-ETHANOLS JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 697 LP - 700 DO - 10.1124/dmd.31.6.697 VL - 31 IS - 6 AU - Jonathan J. Sheng AU - Michael W. Duffel Y1 - 2003/06/01 UR - http://dmd.aspetjournals.org/content/31/6/697.abstract N2 - Hydroxysteroid (alcohol) sulfotransferases catalyze the sulfation of several endogenous steroids and many hydrophobic xenobiotic alcohols. The substrate stereoselectivities of sulfotransferases may be critically important in determining their overall roles in metabolism of drugs, carcinogens, and other xenobiotics. In the present work, stereoselectivity of the human hydroxysteroid sulfotransferase ST2A3 (also variously named as SULT2A1 or human DHEA-ST) was examined through analysis of its catalytic activities with the enantiomers of 1-naphthyl-1-ethanol and 2-naphthyl-1-ethanol. The kcat/Km value for sulfation of the R-(+)-enantiomer of 1-naphthyl-1-ethanol catalyzed by ST2A3 was 3.3 min-1mM-1, whereas the S-(-)-enantiomer was not a substrate for the enzyme. S-(-)-1-naphthyl-1-ethanol did however interact with ST2A3 as an inhibitor of the sulfation of dehydroepiandrosterone. This substrate stereospecificity was not present with the enantiomers of 2-naphthyl-1-ethanol, since both were substrates for the enzyme. Such differences between the sulfation of 1- and 2-naphthyl-1-ethanol are consistent with the importance of steric interactions between the ethanol group and a hydrogen atom at the peri-position (C8) on the naphthyl ring in 1-naphthyl-1-ethanol that combine with the topology of the enzyme's active site to determine stereospecificity. The American Society for Pharmacology and Experimental Therapeutics ER -