PT  - JOURNAL ARTICLE
AU  - Junichi Narukawa
AU  - Hiroki Inoue
AU  - Seiyu Kato
AU  - Hiroshi Yokota
TI  - GLUCURONIDATION OF 1-NAPHTHOL AND EXCRETION INTO THE VEIN IN PERFUSED RAT KIDNEY
AID  - 10.1124/dmd.32.7.758
DP  - 2004 Jul 01
TA  - Drug Metabolism and Disposition
PG  - 758--761
VI  - 32
IP  - 7
4099  - http://dmd.aspetjournals.org/content/32/7/758.short
4100  - http://dmd.aspetjournals.org/content/32/7/758.full
SO  - Drug Metab Dispos2004 Jul 01; 32
AB  - UDP-glucuronosyltransferase is expressed in the proximal convoluted tubular cells of rat kidney. Kidney perfusion with a Krebs-Henseleit buffer containing 1-naphthol was performed to estimate the dynamics and disposition of the glucuronide conjugate formed in the epithelial cells of the renal tubules. When 1-naphthol was injected into the renal artery, and the perfusate from the renal vein was returned to a reservoir and recirculated through the kidney preparation (recirculating perfusion), most of the 1-napthol was immediately excreted into the vein as a glucuronide conjugate and its concentration increased rapidly. In contrast, the 1-napthol glucuronide appeared more slowly in the urine. 1-Naphthol was also injected during the initial 5 min of perfusion under single-pass perfusion conditions (single-pass perfusion) in situ, and the metabolite and parent compound in the venous perfusate and in urine were assayed. Under this condition, most of the 1-naphthol glucuronide was excreted into the renal vein, and not urine. Phenol UDP-glucuronosyltransferase was highly induced in the rat kidney by β-naphthoflavone treatment. Moreover, the amount of 1-naphthol glucuronide excreted in the renal vein was increased 2.7-fold in the perfused kidney of β-naphthoflavone-treated rats, but the amount in the urine was not significantly increased under singlepass perfusion conditions. These results indicate that the kidney can glucuronidate phenolic xenobiotics in epithelial cells of the tubules and excrete the resultant glucuronide into the renal vein. The American Society for Pharmacology and Experimental Therapeutics