PT  - JOURNAL ARTICLE
AU  - Ruchita Kapoor
AU  - Jonathan J. Sheng
TI  - Transfection of Human Prostate Cancer CA-HPV-10 Cells with Cytosolic Sulfotransferase SULT1E1 Affects Estrogen Signaling and Gene Transcription
AID  - 10.1124/dmd.107.017632
DP  - 2008 Feb 01
TA  - Drug Metabolism and Disposition
PG  - 316--321
VI  - 36
IP  - 2
4099  - http://dmd.aspetjournals.org/content/36/2/316.short
4100  - http://dmd.aspetjournals.org/content/36/2/316.full
SO  - Drug Metab Dispos2008 Feb 01; 36
AB  - Human cytosolic sulfotransferase SULT1E1 catalyzes the sulfation of estrogens and estrogenic drugs in human reproductive tissues. Logically, this estrogen-preferring sulfotransferase isoform could play a regulatory role in estrogen signaling activities in human reproductive cells, including the prostate cells. This hypothesis was tested using DNA microarray and real-time reverse transcription-polymerase chain reaction methods in the present work. Potential changes in the transcriptional expression of selected signal transduction-related genes in human prostate cancer CA-HPV-10 cell line after SULT1E1 transfection were examined by DNA microarray methods. Notable changes were observed in the mRNA expression levels of TFRC, a cell membrane transferrin receptor gene, and TMEPAI, a gene encoding a steroid-dependent mRNA product. Expression of TFRC was down-regulated, whereas expression of TMEPAI was up-regulated by SULT1E1 transfection in CA-HPV-10 cells. Data from the current studies also showed that the estrogen-induced estrogen response element activation in CA-HPV-10 cells was repressed after the cells were transfected with SULT1E1. These results indicate that SULT1E1 may function as a transcriptional mediator in human prostate cancer CA-HPV-10 cells. The American Society for Pharmacology and Experimental Therapeutics