TY - JOUR T1 - Pharmacogenetics of Human Carbonyl Reductase 1 (<em>CBR1</em>) in Livers from Black and White Donors JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 400 LP - 407 DO - 10.1124/dmd.108.024547 VL - 37 IS - 2 AU - Vanessa Gonzalez-Covarrubias AU - Jianping Zhang AU - James L. Kalabus AU - Mary V. Relling AU - Javier G. Blanco Y1 - 2009/02/01 UR - http://dmd.aspetjournals.org/content/37/2/400.abstract N2 - Carbonyl reductase 1 (CBR1) reduces the anticancer drug doxorubicin into the cardiotoxic metabolite doxorubicinol. We documented the hepatic expression of CBR1 in samples from white and black donors. Concordance between ethnicity and geographical ancestry was examined with ancestry informative markers. Livers from blacks and whites showed similar CBR1 mRNA levels (CBR1 mRNAblacks = 4.8 ± 4.3 relative -fold versus CBR1 mRNAwhites = 3.6 ± 3.6 relative -fold; p = 0.217). CBR1 protein levels did not differ between both groups (CBR1blacks = 8.0 ± 3.4 nmol/g cytosolic protein versus CBR1whites = 9.0 ± 4.6 nmol/g cytosolic protein; p = 0.347). The CBR1 3′-untranslated region polymorphism 1096G&gt;A was detected in DNA samples from whites (p = 0.875; q = 0.125), and livers with homozygous G/G genotypes showed a trend toward higher CBR1 mRNA levels compared with samples with heterozygous G/A genotypes [CBR1 1096G&gt;A(G/G) = 4.1 ± 4.1 relative -fold versus CBR1 1096G&gt;A(G/A) = 3.0 ± 2.5 relative-fold; p = 0.266]. CBR1 1096G&gt;A genotype status was associated with CBR1 protein levels (p = 0.030) and CBR activity expressed as the rate of synthesis of doxorubicinol (p = 0.028). Our findings warrant further studies to evaluate the impact of CBR1 1096G&gt;A genotype status on the variable pharmacodynamics of anthracycline drugs. The American Society for Pharmacology and Experimental Therapeutics ER -