PT  - JOURNAL ARTICLE
AU  - Lei Guo
AU  - Stacey Dial
AU  - Leming Shi
AU  - William Branham
AU  - Jie Liu
AU  - Jia-Long Fang
AU  - Bridgett Green
AU  - Helen Deng
AU  - Jim Kaput
AU  - Baitang Ning
TI  - Similarities and Differences in the Expression of Drug-Metabolizing Enzymes between Human Hepatic Cell Lines and Primary Human Hepatocytes
AID  - 10.1124/dmd.110.035873
DP  - 2011 Mar 01
TA  - Drug Metabolism and Disposition
PG  - 528--538
VI  - 39
IP  - 3
4099  - http://dmd.aspetjournals.org/content/39/3/528.short
4100  - http://dmd.aspetjournals.org/content/39/3/528.full
SO  - Drug Metab Dispos2011 Mar 01; 39
AB  - In addition to primary human hepatocytes, hepatoma cell lines, and transfected nonhepatoma, hepatic cell lines have been used for pharmacological and toxicological studies. However, a systematic evaluation and a general report of the gene expression spectra of drug-metabolizing enzymes and transporters (DMETs) in these in vitro systems are not currently available. To fill this information gap and to provide references for future studies, we systematically characterized the basal gene expression profiles of 251 drug-metabolizing enzymes in untreated primary human hepatocytes from six donors, four commonly used hepatoma cell lines (HepG2, Huh7, SK-Hep-1, and Hep3B), and one transfected human liver epithelial cell line. A large variation in DMET expression spectra was observed between hepatic cell lines and primary hepatocytes, with the complete absence or much lower abundance of certain DMETs in hepatic cell lines. Furthermore, the basal DMET expression spectra of five hepatic cell lines are summarized, providing references for researchers to choose carefully appropriate in vitro models for their studies of drug metabolism and toxicity, especially for studies with drugs in which toxicities are mediated through the formation of reactive metabolites.