RT Journal Article
SR Electronic
T1 Simultaneous immobilization of cytochrome P-450 and glucuronyltransferase for synthesis of drug metabolites.
JF Drug Metabolism and Disposition
JO Drug Metab Dispos
FD American Society for Pharmacology and Experimental Therapeutics
SP 15
OP 18
VO 9
IS 1
A1 J P Lehman
A1 L Ferrin
A1 C Fenselau
A1 G S Yost
YR 1981
UL http://dmd.aspetjournals.org/content/9/1/15.abstract
AB Cytochrome P-450, NADPH-cytochrome P-450 reductase, and glucuronyltransferase were immobilized simultaneously on cyanogen bromide-activated Sepharose from phenobarbital-induced rabbit liver microsomes. The activity of the P-450 system was demonstrated by the N-demethylation of ethylmorphine and the O-demethylation of p-nitroanisole. p-Nitrophenol produced from the oxidation of p-nitroanisole was conjugated to p-nitrophenyl glucuronide, as evidenced by isolation and characterization of the glucuronide by GC/MS.