RT Journal Article
SR Electronic
T1 Metabolism of sulfanilamide and related drugs in isolated rat and rabbit liver cells.
JF Drug Metabolism and Disposition
JO Drug Metab Dispos
FD American Society for Pharmacology and Experimental Therapeutics
SP 205
OP 207
VO 8
IS 4
A1 E M Suolinna
YR 1980
UL http://dmd.aspetjournals.org/content/8/4/205.abstract
AB Parenchymal and nonparenchymal cells were isolated from rat and rabbit liver by perfusion with collagenase. Pure parenchymal cells were obtained by centrifugation at low speed and nonparenchymal cells were purified by treatment with pronase. Suspensions of parenchymal cells from rat liver metabolized sulfamethazine, sulfanilamide, and p-aminobenzoic acid at rates of 39.0, 75.8, and 173.2 pmol/min/10(6) cells, respectively, but did not metabolize sulfadiazine. Rabbit liver parenchymal cells gave somewhat higher activities and also metabolized sulfadiazine. Nonparenchymal cells from both rat and rabbit liver failed to metabolize any of the compounds.