TY - JOUR T1 - In Vivo-Formed versus Preformed Metabolite Kinetics of <em>trans</em>-Resveratrol-3-sulfate and <em>trans</em>-Resveratrol-3-glucuronide JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 1993 LP - 2001 DO - 10.1124/dmd.112.046417 VL - 40 IS - 10 AU - Satish Sharan AU - Otito F. Iwuchukwu AU - Daniel J. Canney AU - Cheryl L. Zimmerman AU - Swati Nagar Y1 - 2012/10/01 UR - http://dmd.aspetjournals.org/content/40/10/1993.abstract N2 - Metabolites in safety testing have gained a lot of attention recently. Regulatory agencies have suggested that the kinetics of preformed and in vivo-formed metabolites are comparable. This subject has been a topic of debate. We have compared the kinetics of in vivo-formed with preformed metabolites. trans-3,5,4′-Trihydroxystilbene [trans-resveratrol (RES)] and its two major metabolites, resveratrol-3-sulfate (R3S) and resveratrol-3-glucuronide (R3G) were used as model substrates. The pharmacokinetics (PK) of R3S and R3G were characterized under two situations. First, the pharmacokinetics of R3S and R3G were characterized (in vivo-formed metabolite) after administration of RES. Then, synthetic R3S and R3G were administered (preformed metabolite) and their pharmacokinetics were characterized. PK models were developed to describe the data. A three-compartment model for RES, a two-compartment model for R3S (preformed), and an enterohepatic cycling model for R3G (preformed) was found to describe the data well. These three models were further combined to build a comprehensive PK model, which was used to perform simulations to predict in vivo-formed metabolite kinetics. Comparisons were made between in vivo-formed and preformed metabolite kinetics. Marked differences were observed in the kinetics of preformed and in vivo-formed metabolites. ER -