@article {Rock1341, author = {Brooke M. Rock and Mark E. Tometsko and Sonal K. Patel and Kevin J. Hamblett and William C. Fanslow and Dan A. Rock}, title = {Intracellular Catabolism of an Antibody Drug Conjugate with a Noncleavable Linker}, volume = {43}, number = {9}, pages = {1341--1344}, year = {2015}, doi = {10.1124/dmd.115.064253}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {Antibody drug conjugates are emerging as a powerful class of antitumor agents with efficacy across a range of cancers; therefore, understanding the disposition of this class of therapeutic is crucial. Reported here is a method of enriching a specific organelle (lysosome) to understand the catabolism of an anti-CD70 Ab-MCC-DM1, an antibody drug conjugate with a noncleavable linker. With such techniques a higher degree of concentration-activity relationship can be established for in vitro cell lines; this can aid in understanding the resultant catabolite concentrations necessary to exert activity.}, issn = {0090-9556}, URL = {https://dmd.aspetjournals.org/content/43/9/1341}, eprint = {https://dmd.aspetjournals.org/content/43/9/1341.full.pdf}, journal = {Drug Metabolism and Disposition} }