TY - JOUR T1 - Intracellular Catabolism of an Antibody Drug Conjugate with a Noncleavable Linker JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 1341 LP - 1344 DO - 10.1124/dmd.115.064253 VL - 43 IS - 9 AU - Brooke M. Rock AU - Mark E. Tometsko AU - Sonal K. Patel AU - Kevin J. Hamblett AU - William C. Fanslow AU - Dan A. Rock Y1 - 2015/09/01 UR - http://dmd.aspetjournals.org/content/43/9/1341.abstract N2 - Antibody drug conjugates are emerging as a powerful class of antitumor agents with efficacy across a range of cancers; therefore, understanding the disposition of this class of therapeutic is crucial. Reported here is a method of enriching a specific organelle (lysosome) to understand the catabolism of an anti-CD70 Ab-MCC-DM1, an antibody drug conjugate with a noncleavable linker. With such techniques a higher degree of concentration-activity relationship can be established for in vitro cell lines; this can aid in understanding the resultant catabolite concentrations necessary to exert activity. ER -