TY - JOUR T1 - Regional Proteomic Quantification of Clinically Relevant Non-Cytochrome P450 Enzymes along the Human Small Intestine JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 528 LP - 536 DO - 10.1124/dmd.120.090738 VL - 48 IS - 7 AU - Haeyoung Zhang AU - Chris Wolford AU - Abdul Basit AU - Albert P. Li AU - Peter W. Fan AU - Bernard P. Murray AU - Ryan H. Takahashi AU - S. Cyrus Khojasteh AU - Bill J. Smith AU - Kenneth E. Thummel AU - Bhagwat Prasad Y1 - 2020/07/01 UR - http://dmd.aspetjournals.org/content/48/7/528.abstract N2 - Current challenges in accurately predicting intestinal metabolism arise from the complex nature of the intestine, leading to limited applicability of available in vitro tools as well as knowledge deficits in intestinal physiology, including enzyme abundance. In particular, information on regional enzyme abundance along the small intestine is lacking, especially for non–cytochrome P450 enzymes such as carboxylesterases (CESs), UDP-glucuronosyltransferases (UGTs), and sulfotransferases (SULTs). We used cryopreserved human intestinal mucosa samples from nine donors as an in vitro surrogate model for the small intestine and performed liquid chromatography tandem mass spectrometry–based quantitative proteomics for 17 non–cytochrome P450 enzymes using stable isotope–labeled peptides. Relative protein quantification was done by normalization with enterocyte marker proteins, i.e., villin-1, sucrase isomaltase, and fatty acid binding protein 2, and absolute protein quantification is reported as picomoles per milligram of protein. Activity assays in glucuronidations and sequential metabolisms were conducted to validate the proteomics findings. Relative or absolute quantifications are reported for CES1, CES2, five UGTs, and four SULTs along the small intestine: duodenum, jejunum, and ileum for six donors and in 10 segments along the entire small intestine (A–J) for three donors. Relative quantification using marker proteins may be beneficial in further controlling for technical variabilities. Absolute quantification data will allow for scaling factor generation and in vivo extrapolation of intestinal clearance using physiologically based pharmacokinetic modeling.SIGNIFICANCE STATEMENT Current knowledge gaps exist in intestinal protein abundance of non–cytochrome P450 enzymes. Here, we employ quantitative proteomics to measure non–cytochrome P450 enzymes along the human small intestine in nine donors using cryopreserved human intestinal mucosa samples. Absolute and relative abundances reported here will allow better scaling of intestinal clearance. ER -